She won the title at Kemnay Golf Club, Aberdeenshire today after long-time leader Alex Glennie (Kilmarnock Barassie) dropped four shots to par over the last four holes.
Glennie, well clear of the field, had a double bogey 7 at the 15th and bogeys at the 16th and 17th for a six-over-par round of 79 (39-40) which gave her a two-round final total of eight-over 154.
Bartlett matched that aggregate by birdieing the 17th on her way to a one-under 72 (37-35).
Glennie's downward spiral continued when she lost the sudden-death play-off to Bartlett at the first extra hole.
Tain sisters Mary Smith (81 for 158) and Anne Ryan (80 for 161) finished third and joint fifth to give the far North three players in the top six - a grand performance.
The leading 16 players go on to contest the seniors' match-play championship over the next two days.
Linda Urquhart (Banchory) was the 16th qualifier with an 85 for 168 - pipping former Scottish seniors champion Karen Ballantyne (Craigmillar Park) (83 for 169) for the final place.
SCOTTISH SENIOR WOMEN'S AMATEUR STROKE-PLAY CHAMPIONSHIP
Kemnay GC, Aberdeenshire
MATCH-PLAY QUALIFIERS
Par 146 (2x73)
154 Alison Bartlett (Royal Dornoch) 82 72, Alex Glennie (Kilmarnock Barassie) (Bartlett won sudden-death play-off at first extra hole).
158 Mary Smith (Tain) 77 81.
160 Elaine Allison (Stirling) 82 78.
161 Anne Ryan (Tain) 81 80, Helen Faulds (Douglas Park) 80 81.
163 Kathleen Sutherland (Royal Montrose) 80 83.
164 Anne Telfer (Milngavie) 83 81, Pamela Williamson (Baberton) 82 82, Heather Anderson (Blairgowrie) 81 83
165 Fiona Hunter (Baberton) 84 81, Jill Harrison (Cruden Bay) 77 88
166 Janice Paterson (Drumpellier) 81 85.
167 Karen Burns (Bathgate) 87 80, Margaret Tough (Falkirk) 87 80.
168 Linda Urquhart (Banchory) 83 85.
DID NOT QUALIFY
169 Karen Ballantyne (Craigmillar Park) 86 83.
170 Lorraine Campbell (Cardross) 87 83, Sue Penman (Gullane Ladies) 84 86, Gillian Wallace (Blairgowrie) 84 86, May Hughes (Lanark) 83 87
172 Anne Brownie (Gullane Ladies) 87 85m Barbara Biggart (North Berwick) 86 86, Lynne Terry (Cruden Bay) 83 89.
To see all the final scores --- CLICK HERE
CHAMPIONSHIP DRAW
